XAS Study of a Venom Metalloprotein

LI Shu-Jun; ZHAO Wei; CHU Wang-Sheng; NIU Li-Wen; TENG Mai-Kun; HU Tian-Dou; XIE Ya-Ning; WU Zi-Yu

Chinese Physics C> 2003, Vol. 27> Issue(S1) : 19-22

XAS Study of a Venom Metalloprotein

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 1000392 University of Science and Technology of China,Hefei 230026,China

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Abstract：
In this paper,we probed the Acutolysin A—a Zinc protein purified from venom-by XAS technique,and its XAS curves in solution state of different pH values,were obtained.It was confirmed that the intense X-Rays didnt damage the samples during the experiment,and the curves among different pH values did present some differences.Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal,we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution.At the same time,XANES analysis result could not reconstruct the experimental EXAFS curve either,because the PDB cluster used in the XANES calculation is free of Hydrogen atoms,therefore,a Hydrogen-contained cluster might be essential to the good fitting of XANES.

References

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Hasnain S S, Hodgson K 0. J. Synchrolron Rad. , 1999, 6 : 8522 Cranier S P. X-Ray Ahsorplion : Principles, Applicalions , Techniques of EXAFS, SEXAFS and XANES. Koningsherger D C and Prins R, eds, New York: Wiley , 1988. 2573 Scott R A. Slructural and Resonance Techniques in Biological Research.( D. L. Rousseau, ed. ) , Orlando, Florida: Academic Press, 1984. 2954 Powers L. Biochini. Biophys. Acta, 1982, 1 :6835 Rehr J J, Ankudinov A L. J. Synchrotron Rad. , 2003 , 10: 436 WU Z Y et al. Physica Scripla, 2003 (in press)7 Naloli C R, Benfatto M, Longa S D el al. J. Synchrotron Rad. ,2003, 10 : 268 Hite L A , Shannon J D, Rjarnason J B et al. Biochemistry, 1992, 31(27 ) : 62039 Hite L A , Jia I. G. , Bjarnason J B et al. Arch. Biochem. Biophys. ,1994, 308(1): 18210 GONG W, ZHU X , LIU S et al. J. Mol. Biol. , 1998, 283 ( 3 ) :65711 XU X, WANG C, LIU J et al. Toxicon, 1981 , 19(5) : 63312 Hasnain S S, Strange R W. J. Synchrotron Rad. , 2003, 10:9

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LI Shu-Jun, ZHAO Wei, CHU Wang-Sheng, NIU Li-Wen, TENG Mai-Kun, HU Tian-Dou, XIE Ya-Ning and WU Zi-Yu. XAS Study of a Venom Metalloprotein[J]. Chinese Physics C, 2003, 27(S1): 19-22.

LI Shu-Jun, ZHAO Wei, CHU Wang-Sheng, NIU Li-Wen, TENG Mai-Kun, HU Tian-Dou, XIE Ya-Ning and WU Zi-Yu. XAS Study of a Venom Metalloprotein[J]. Chinese Physics C, 2003, 27(S1): 19-22. shu

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Received: 2003-11-21
Revised: 1900-01-01

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沈阳化工大学材料科学与工程学院沈阳 110142

XAS Study of a Venom Metalloprotein

Corresponding author: LI Shu-Jun,

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 1000392 University of Science and Technology of China,Hefei 230026,China

Received Date: 2003-11-21
Accepted Date: 1900-01-01
Available Online: 2003-01-02

Abstract: In this paper,we probed the Acutolysin A—a Zinc protein purified from venom-by XAS technique,and its XAS curves in solution state of different pH values,were obtained.It was confirmed that the intense X-Rays didnt damage the samples during the experiment,and the curves among different pH values did present some differences.Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal,we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution.At the same time,XANES analysis result could not reconstruct the experimental EXAFS curve either,because the PDB cluster used in the XANES calculation is free of Hydrogen atoms,therefore,a Hydrogen-contained cluster might be essential to the good fitting of XANES.