Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

CHEN Xiao-Ming; TAN Bi-Sheng; ZHENG Chun; ZHANG Jian-Guo; WANG Dan; CHU Shi-Jin; REN Zheng-Long

Chinese Physics C> 2007, Vol. 31> Issue(10) : 972-977

Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

Southwest University of Science and Technology, Mianyang 621010, China2 Sichuan Agriculture University, Yaan 625000, China3 Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

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Abstract：
The content of Bacillus substilis var. niger (ATCC 9372) DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage (PR), DNA breakage level (L), average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.

References

[1]

. XIA Shou-Xuan. Radiobiology. Beijing: The Academy of Military Medical Sciences Press, 1998. 190-198(in Chi-nese) (夏寿萱主编.放射生物学.北京:军事医学科学出版社,1998.190-198)2. Pinto M, Prise K M, Michael B D. Int. J Radiat Biol., 2002,78(5): 375-3883. ZHENG Chun, LI Jian-Sheng, WU Jian-Hua. Nuclear Electronics Detection Technology, 2001, 21(4): 307-309(in Chi-nese) (郑春，李建胜，吴建华.核电子学与探测技术，2001, 21(4):307-309)4. ZANG Li-Hui, WEI Zhi-Yong, LI Ming et al. Chin. JHemorh, 2005, 15(2): 193-196(in Chi-nese) (臧黎慧，魏志勇，李明等.中国血液流变学杂志，2005, 15(2):193-196)5. ZHOU Guang-Ming, LI Wen-Jian, WANG Ju-Fang et al.Journal of Radiation Research and Radiation Processing,2001, 19(4): 289-294(in Chi-nese) (周光明，李文建，王菊芳等.辐射研究与辐射工艺学报，200119(4): 289-294)6. SUI Li, ZHAO Kui, NI Mei-Nan et al. HEP NP, 2004,28(10): 1126-1130(in Chi-nese)(隋丽，赵葵，倪嵋楠等.高能物理与核物理，2004, 28(10)1126-1130) 7. Nikjoo H, Nell P O, Wilson W E et al. Radiation Research,2001, 156: 577-5838. Rydberg B. Radiation Research, 1996, 145: 200-2099. Koufen P, Brdiczka D, Stark G. Int. J. Radiat. Biol., 2000,76(5): 625-63110. WEI Zeng-Quan, ZHOU Guang-Ming, WANG Ju-Fang etal. In: Book of Abstracts in 6th Workshop on Heavy-Charged Particles in Biology and Medicine. Carbon IonInduced DNA Double-Strand Breaks in Melanophore B16.Baveno, Italy. 1997. 711. Sutherland B M, Bennett P V, Sidorkina O et al. Biochemistry,2000, 39(27): 8026-803112. Newman H C, Prise K M, Fclkard M et al. Int. J. Radiat.Biol., 1997, 71(4): 347-36313. Lobrich M, Cooper P K, Rydberg B. Int. J. Radiat. Biol.,1996, 70(2): 493-50314. ZHOU Guang-Ming, LI Wen-Jian, WANG Ju-Fang et al.Acta Biophysica Sinica, 2000, 16(1): 139-145(in Chi-nese)(周光明，李文建，王菊芳等.生物物理学报，2000, 16(1):139-145)15. ZHOU Guang-Ming, LI Wen-Jian, Gao Qing-Xiang et al.Nuclear Physics Review, 2002, 20(1): 52-54(in Chi-nese)(周光明，李文建，高清祥等.原子核物理评论，2002, 20(1):52-54) 16. Schmid E, Regulla D, Guldbakke S et al. Radiation Research,2002, 157: 453-46017. Hacker U B, Kohnlein W, Kronholz H L et al. RadiationResearch, 2000, 153(6): 734-74218. Lowy R J, Vavrina G A, Labarre D D. Antiviral Research,2001, 52: 261-273

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[2]	HU Shu-Xin , LI Da-Peng , MAI Zhen-Hong , LI Ming . Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes. Chinese Physics C, 2005, 29(S1): 4-7.
[3]	SUI Li , ZHAO Kui , NI Mei-Nan , GUO Ji-Yu , LUO Hong-Bing , MEI Jun-Ping , LU Xiu-Qin , ZHOU Ping . Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions. Chinese Physics C, 2004, 28(10): 1126-1130.
[4]	LIU Jian-Feng , JIA Min , ZHAO Wei-Juan . Study on the Giant Dipole Resonance Model for γ Production Mechanisms Induced by Fast Nutrons in the Nuclear Mass Region 180≤A≤210. Chinese Physics C, 2003, 27(5): 430-435.
[5]	LIANG Ling , GU Mu , DUAN Yong , MA Xiao-Hui , LIU Feng-Song , WU Xiang-Hui , QIU Long-Qing , CHEN Ming-Nan , LIAO Jing-Ying , SHEN Ding-Zhong , ZHANG Xin , GONG Bo , XUE Xuan-Ping , , . Study on Irradiation Damage of Bi-Doped PbWO₄ Crystal. Chinese Physics C, 2003, 27(6): 526-531.
[6]	LIU Jian-Feng , ZHAO Wei-Juan . Stuty on the Process of the Fast Neutron Radiative Capture for ⁴⁰Ca and ²⁰⁸Pb. Chinese Physics C, 2002, 26(11): 1158-1165.
[7]	ZHAO WeiJuan , LIU JianFeng , HUO YuKun . Resonance Phenomenon in the Fast Neutron Radiative Capture Reaction for ¹²C. Chinese Physics C, 2000, 24(12): 1137-1141.
[8]	Ye Bangjiao , Zhou Xianyi , Han Rongdian , Kasugai Yoshimi , Ikeda Yujiro . Backward Sputtering induced by Fast Neutron. Chinese Physics C, 1999, 23(8): 760-764.
[9]	Li Zuhao , He Jingtang , Lu Yusheng , Chen Duanbao , Bian Jianguo , Zhu Guoyi , Tang Xiaowei . Study on Radiation Damage of Lead Tungstate Crystals. Chinese Physics C, 1999, 23(12): 1148-1151.
[10]	Zhu Yongsheng , Chen Yu , Shi Huanhang , Yu Chunxu , Liao Yihua , Xia Xiaomi , Lai Yuanfen , Zhang Zhuxiang , Zhong Xuechu , Zhang Caidi , Zheng Linsheng , Zhang Changchun , Chen Gang , Ren Shaoxia , Zheng Yanning , Liu Hu . Experimental Studies on Radiation Damage of China Made CsI(TI) Crystals. Chinese Physics C, 1998, 22(10): 865-872.
[11]	He Jingtang , Mao Yufang , Dong Xiaoli , Chen Duanbao , Li Zuhao . Experimental Studies on Radiation Damages of CsI(Tl) Crystals. Chinese Physics C, 1997, 21(1): 21-24.
[12]	Mao Huishun , Shi Huanzhang , Liu Nianzong , Zhang Zhuxiang , Zhang Caidi , Zheng Linsheng , Zhou Yongshen , Hu Lidong , Zhao Xiaojian , Zhong Xuechu , Tan Yiping , Han Shiwen . Further Study of Radiation Damage of Tile/Fiber Scintillator Calorimeter. Chinese Physics C, 1994, 18(10): 870-878.
[13]	CHEN Yu , DAI Chang-Jiang , GU Yi-Fan , LEI Zhuan-Heng , LI Jian-Ping , LIU Shu-Dong , SHAO Bei-Bei , TANG Yue-Li . Study on Background Radiation at Beijing Electron Positron Collider and Radiation Damage of High-Z Scintillation Crystals. Chinese Physics C, 1993, 17(8): 673-679.
[14]	MAO Hui-Shun , WANG Guo-Liang , LIU Nian-Zong , ZHANG Ge , ZHANG Zhu-Xiang , ZHANG Liang-Sheng , ZHANG Cai-Di , ZHENG Lin-Sheng , ZHOU Yong-Shen , HU Li-Dong , ZHAO Xiao-Jian , ZHONG Xue-Chu , TAN Yi-Ping , HAN Shi-Wen . Study of Radiation Damage of Tile/Fiber Scintillator Calorimeter. Chinese Physics C, 1992, 16(10): 876-885.
[15]	MU Yun-Shan , XU Hai-Shan , XIANG Zheng-Yu , LI Ye-Xiang , WANG Shi-Ming , LIU Jian-Feng . Fast Neutron Capture Cross Section of Natural Molybdenum. Chinese Physics C, 1991, 15(1): 66-70.
[16]	CHEN Jia-Chao , CHEN Ke-Qin , MA Feng . The Calculation of Depth Distribution for Swift Ion-Radiated damage Energy. Chinese Physics C, 1991, 15(1): 59-65.
[17]	ZHU Guo-Yi , HE Jing-Tang , GU Yi-Fan , QIAN Zhong-Min , CHU Guo-Zhu . STUDY ON RADIATION DAMAGE OF CHINESE-MADE HIGH-Z SCINTILLATION CRYSTALS. Chinese Physics C, 1990, 14(1): 8-15.
[18]	XIANG Zheng-Yu , XU Hai-Shan , LI Ye-Xiang , MU Yun-Shan , WANG Shi-Ming , LIU Jian-Feng . FAST NEUTRON CAPTURE CROSS SECTIONS OF FISSION PRODUCT NUCLIDE Eu. Chinese Physics C, 1989, 13(10): 938-941.
[19]	LU HAN-LIN , WANG DA-HAI , XIA YI-JUN , CUI YUN-FEN , G CHEN PAO-LIN . EXCITATION CURVES FOR SOME REACTIONS OF Al、Ti、V AND I. Chinese Physics C, 1979, 3(1): 88-96.
[20]	NEUTRON PHYSICS GROUP OF NUCLEAR PHYSICS LABORATORY . ELASTIC SCATTERING OF FAST NEUTRONS FROM Fe, Cu AND Pb. Chinese Physics C, 1978, 2(5): 450-460.

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CHEN Xiao-Ming, TAN Bi-Sheng, ZHENG Chun, ZHANG Jian-Guo, WANG Dan, CHU Shi-Jin and REN Zheng-Long. Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation[J]. Chinese Physics C, 2007, 31(10): 972-977.

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Received: 2006-10-19
Revised: 2007-03-22

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

Corresponding author: REN Zheng-Long,

Southwest University of Science and Technology, Mianyang 621010, China2 Sichuan Agriculture University, Yaan 625000, China3 Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

Received Date: 2006-10-19

Accepted Date: 2007-03-22

Available Online: 2007-10-05

Abstract: The content of Bacillus substilis var. niger (ATCC 9372) DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage (PR), DNA breakage level (L), average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.

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Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

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